Lymphocyte surface thiol levels

Lymphocyte surface thiol levels.

Recent studies have implicated reduced thiols (cysteine -SH) in the function of individual cell surface proteins. Studies presented here demonstrate that the overall level of reduced thiols on cell surface molecules differs on individual subsets of peripheral blood mononuclear cells and that these levels can be manipulated in vitro by altering the level of intracellular glutathione (iGSH). To q...

Gold Solid Surface Thiol Oligo Conjugation

Cell surface Glut1 levels distinguish human CD4 and CD8 T lymphocyte subsets with distinct effector functions.

CD4 and CD8 T lymphocyte activation requires the generation of sufficient energy to support new biosynthetic demands. Following T cell receptor (TCR) engagement, these requirements are met by an increased glycolysis, due, at least in part, to induction of the Glut1 glucose transporter. As Glut1 is upregulated on tumor cells in response to hypoxia, we assessed whether surface Glut1 levels regula...

Effect of N-acetyl cysteine on thiol levels.

Effect of N-acetyl cysteine on thiol levels We read with interest the article by Dr Bridge-man and colleagues on the effect of oral N-acetyl cysteine (NAC) on thiol levels in epithelial lining fluid (ELF) and lung tissue (July 1994;49:670-5). Their suggestion that NAC may not be the drug of choice to enhance the glutathione antioxidant potential of the lungs in chronic obstructive pulmonary dis...

The platelet-surface thiol isomerase enzyme ERp57 modulates platelet function

BACKGROUND Thiol isomerases are a family of endoplasmic reticulum enzymes which orchestrate redox-based modifications of protein disulphide bonds. Previous studies have identified important roles for the thiol isomerases PDI and ERp5 in the regulation of normal platelet function. AIM Recently, we demonstrated the presence of a further five thiol isomerases at the platelet surface. In this rep...